between two datasets using Discrete Wavelet Transfoms
execute_dwt_IvC_all.pl $inputFile1 $inputFile2 $outputFile1 $outputFile2
.. class:: infomark
**What it does**
This program generates plots and computes table matrix of second moments, p-values, and test orientations at multiple scales for the correlation between the occurrences of features in one dataset and their occurrences in another using multiscale wavelet analysis technique.
The program assumes that the user has two sets of DNA sequences, S1 and S1, each of which consists of one or more sequences of equal length. Each sequence in each set is divided into the same number of multiple intervals n such that n = 2^k, where k is a positive integer and k >= 1. Thus, n could be any value of the set {2, 4, 8, 16, 32, 64, 128, ...}. k represents the number of scales.
The program has two input files obtained as follows:
For a given set of features, say motifs, the user counts the number of occurrences of each feature in each interval of each sequence in S1 and S1, and builds two tabular files representing the count results in each interval of S1 and S1. These are the input files of the program.
The program gives two output files:
- The first output file is a TABULAR format file representing the second moments, p-values, and test orientations for each feature at each scale.
- The second output file is a PDF file consisting of as many figures as the number of features, such that each figure represents the values of the second moment for that feature at every scale.
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.. class:: warningmark
**Note**
In order to obtain empirical p-values, a random perumtation test is implemented by the program, which results in the fact that the program gives slightly different results each time it is run on the same input file.
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**Example**
Counting the occurrences of 5 features (motifs) in 16 intervals (one line per interval) of the DNA sequences in S1 gives the following tabular file::
deletionHoptspot insertionHoptspot dnaPolPauseFrameshift topoisomeraseCleavageSite translinTarget
226 403 416 221 1165
236 444 380 241 1223
242 496 391 195 1116
243 429 364 191 1118
244 410 371 236 1063
230 386 370 217 1087
275 404 402 214 1044
265 443 365 231 1086
255 390 354 246 1114
281 384 406 232 1102
263 459 369 251 1135
280 433 400 251 1159
278 385 382 231 1147
248 393 389 211 1162
251 403 385 246 1114
239 383 347 227 1172
And counting the occurrences of 5 features (motifs) in 16 intervals (one line per interval) of the DNA sequences in S2 gives the following tabular file::
deletionHoptspot insertionHoptspot dnaPolPauseFrameshift topoisomeraseCleavageSite translinTarget
235 374 407 257 1159
244 356 353 212 1128
233 343 322 204 1110
222 329 398 253 1054
216 325 328 253 1129
257 368 352 221 1115
238 360 346 224 1102
225 350 377 248 1107
230 330 365 236 1132
241 389 357 220 1120
274 354 392 235 1120
250 379 354 210 1102
254 329 320 251 1080
221 355 406 279 1127
224 330 390 249 1129
246 366 364 218 1176
We notice that the number of scales here is 4 because 16 = 2^4. Runnig the program on the above input files gives the following output:
The first output file::
motif 1_moment2 1_pval 1_test 2_moment2 2_pval 2_test 3_moment2 3_pval 3_test 4_moment2 4_pval 4_test
deletionHoptspot 0.8751 0.376 L 1.549 0.168 R 0.6152 0.434 L 0.5735 0.488 R
insertionHoptspot 0.902 0.396 L 1.172 0.332 R 0.6843 0.456 L 1.728 0.213 R
dnaPolPauseFrameshift 1.65 0.013 R 0.267 0.055 L 0.1387 0.124 L 0.4516 0.498 L
topoisomeraseCleavageSite 0.7443 0.233 L 1.023 0.432 R 1.933 0.155 R 1.09 0.3 R
translinTarget 0.5084 0.057 L 0.8219 0.446 L 3.604 0.019 R 0.4377 0.492 L
The second output file:
.. image:: ../static/operation_icons/dwt_IvC_1.png
.. image:: ../static/operation_icons/dwt_IvC_2.png
.. image:: ../static/operation_icons/dwt_IvC_3.png
.. image:: ../static/operation_icons/dwt_IvC_4.png
.. image:: ../static/operation_icons/dwt_IvC_5.png