Predicts potentially antigenic regions of a protein sequence, using the method of Kolaskar and Tongaonkar. emboss antigenic -sequence $input1 -outfile $out_file1 -minlen $minlen -rformat2 $out_format1 -auto You can view the original documentation here_. .. _here: http://emboss.sourceforge.net/apps/release/5.0/emboss/apps/antigenic.html