Calculates DNA RNA/DNA melting temperature emboss emboss_single_outputfile_wrapper.pl dan -sequence $input1 -windowsize $window -goutfile $out_file1 -graph png -plot $plot1 -shiftincrement $shift -dnaconc $dnaconc -saltconc $saltconc -product $product -formamide $formamide -mismatch $mismatch -prodlen $prodlen -thermo $thermo -temperature $temperature -rna $rna -outfile $out_file1 -auto On query Window Size Step size (shift increment) DNA Concentration (nM) Salt concentration (mM) Output the DeltaG, DeltaH and DeltaS values Yes No Temperature at which to calculate the DeltaG, DeltaH and DeltaS values Sequence is RNA No Yes Include percent formamide, percent of mismatches allowed and product length No Yes Formamide concentration (nM) Percent mismatch to be used in calculations Product length to be used in calculations Create a graph No Yes You can view the original documentation here_. .. _here: http://emboss.sourceforge.net/apps/release/5.0/emboss/apps/dan.html