Finds PEST motifs as potential proteolytic cleavage sites emboss emboss_single_outputfile_wrapper.pl epestfind -sequence $input1 -goutfile $ofile2 -outfile $ofile1 -window $window -order $order -potential $potential -poor $poor -invalid $invalid -map $map -graph png -auto Sequence Minimal distance between positively charged amino acids Sort by Score Length Position Threshold value to discriminate weak from potential PEST motifs. Decide whether potential PEST motifs should be printed Yes No Decide whether poor PEST motifs should be printed Yes No Decide whether invalid PEST motifs should be printed No Yes Decide whether PEST motifs should be mapped to sequence Yes No You can view the original documentation here_. .. _here: http://emboss.sourceforge.net/apps/release/5.0/emboss/apps/epestfind.html