Mutate sequence beyond all recognition emboss msbar -sequence $input1 -outseq $out_file1 -count $count -point $point -block $block -codon $codon -inframe $inframe -minimum $minimum -maximum $maximum -osformat2 $out_format1 -auto Sequence 1 Number of times to perform the mutation operations Types of point mutations to perform None Any of the following Insertions Deletions Changes Duplications Moves Types of block mutations to perform None Any of the following Insertions Deletions Changes Duplications Moves Types of codon mutations to perform. These are only done if the sequence is nucleic None Any of the following Insertions Deletions Changes Duplications Moves Do 'codon' and 'block' operations in frame No Yes Minimum size for a block mutation Maximum size for a block mutation Output Sequence File Format FASTA (m) ACeDB (m) ASN.1 (m) Clustal (m) CODATA (m) EMBL (m) Fitch (m) Wisconsin Package GCG 9.x and 10.x (s) GENBANK (m) GFF (m) Hennig86 (m) Intelligenetics (m) Jackknifer (m) Jackknifernon (m) Mega (m) Meganon (m) Wisconsin Package GCG's MSF (m) NBRF (PIR) (m) NCBI style FASTA (m) Nexus/PAUP (m) Nexusnon/PAUPnon (m) PHYLIP interleaved (m) PHYLIP non-interleaved (m) SELEX (m) Staden (s) DNA strider (m) SwisProt entry (m) Plain sequence (s) Treecon (m)

.. class:: warningmark The input dataset needs to be sequences. ----- You can view the original documentation here_. .. _here: http://emboss.sourceforge.net/apps/release/5.0/emboss/apps/msbar.html