#Dan Blankenberg import sys def main(): input_filename = sys.argv[1] output_filename = sys.argv[2] identifier_col = int( sys.argv[3] ) - 1 sequence_col = int( sys.argv[4] ) - 1 quality_col = int( sys.argv[5] ) - 1 max_col = max( identifier_col, sequence_col, quality_col ) num_reads = None fastq_read = None skipped_lines = 0 out = open( output_filename, 'wb' ) for num_reads, line in enumerate( open( input_filename ) ): fields = line.rstrip( '\n\r' ).split( '\t' ) if len( fields ) > max_col: out.write( "@%s\n%s\n+\n%s\n" % ( fields[identifier_col], fields[sequence_col], fields[quality_col] ) ) else: skipped_lines += 1 out.close() if num_reads is None: print "Input was empty." else: print "%i tabular lines were written as FASTQ reads. Be sure to use the FASTQ Groomer tool on this output before further analysis." % ( num_reads + 1 - skipped_lines ) if __name__ == "__main__": main()