fastx_toolkitzcat -f '$input' | fastx_trimmer -v -f $first -l $last -o $output
**What it does**
This tool trims (cut bases from) sequences in a FASTA/Q file.
--------
**Example**
Input Fasta file (with 36 bases in each sequences)::
>1-1
TATGGTCAGAAACCATATGCAGAGCCTGTAGGCACC
>2-1
CAGCGAGGCTTTAATGCCATTTGGCTGTAGGCACCA
Trimming with First=1 and Last=21, we get a FASTA file with 21 bases in each sequences (starting from the first base)::
>1-1
TATGGTCAGAAACCATATGCA
>2-1
CAGCGAGGCTTTAATGCCATT
Trimming with First=6 and Last=10, will generate a FASTA file with 5 bases (bases 6,7,8,9,10) in each sequences::
>1-1
TCAGA
>2-1
AGGCT
------
This tool is based on `FASTX-toolkit`__ by Assaf Gordon.
.. __: http://hannonlab.cshl.edu/fastx_toolkit/