of high quality score reads short_reads_figure_high_quality_length.py $input1 $output1 $input2 rpy

.. class:: warningmark To use this tool, your dataset needs to be in the *Quality Score* format. Click the pencil icon next to your dataset to set the datatype to *Quality Score* (see below for examples). ----- **What it does** This tool takes Quality Files generated by Roche (454), Illumina (Solexa), or ABI SOLiD machines and builds a histogram of lengths of high quality reads. ----- **Examples of Quality Data** Roche (454) or ABI SOLiD data:: >seq1 23 33 34 25 28 28 28 32 23 34 27 4 28 28 31 21 28 Illumina (Solexa) data:: -40 -40 40 -40 -40 -40 -40 40 ----- **Note** - Quality score data:: >seq1 23 33 34 25 28 28 28 32 23 34 27 4 28 28 31 21 28 - If the threshold is set to 20: - a low quality score 4 in the middle separated two segments of lengths 11 and 5. - The histogram will be built based on the numbers (11, 5). - For Illumina (Solexa) data, only the maximal of the 4 values will be used.