#!/usr/bin/env python """ Split into windows. usage: %prog input size out_file -l, --cols=N,N,N,N: Columns for chrom, start, end, strand in file """ import sys, re, os from galaxy import eggs import pkg_resources; pkg_resources.require( "bx-python" ) from bx.cookbook import doc_optparse from galaxy.tools.util.galaxyops import * def stop_err( msg ): sys.stderr.write( msg ) sys.exit() def main(): # Parsing Command Line here options, args = doc_optparse.parse( __doc__ ) try: chr_col_1, start_col_1, end_col_1, strand_col_1 = parse_cols_arg( options.cols ) inp_file, winsize, out_file, makesliding, offset = args winsize = int(winsize) offset = int(offset) makesliding = int(makesliding) if strand_col_1 <= 0: strand = "+" #if strand is not defined, default it to + except: stop_err( "Data issue, click the pencil icon in the history item to correct the metadata attributes of the input dataset." ) fo = open(out_file,'w') skipped_lines = 0 first_invalid_line = 0 invalid_line = None if offset == 0: makesliding = 0 for i, line in enumerate( file( inp_file ) ): line = line.strip() if line and line[0:1] != "#": try: elems = line.split('\t') if strand_col_1 != -1: strand = elems[strand_col_1] start = int(elems[start_col_1]) end = int(elems[end_col_1]) if makesliding == 0: numwin = (end - start)/winsize else: numwin = (end - start)/offset if numwin > 0: for win in range(numwin): elems_1 = elems elems_1[start_col_1] = str(start) elems_1[end_col_1] = str(start + winsize) fo.write( "%s\n" % '\t'.join( elems_1 ) ) if makesliding == 0: start = start + winsize else: start = start + offset if start+winsize > end: break except: skipped_lines += 1 if not invalid_line: first_invalid_line = i + 1 invalid_line = line fo.close() if makesliding == 1: print 'Window size=%d, Sliding=Yes, Offset=%d' %(winsize, offset) else: print 'Window size=%d, Sliding=No' %(winsize) if skipped_lines > 0: print 'Skipped %d invalid lines starting with #%d: "%s"' % ( skipped_lines, first_invalid_line, invalid_line ) if __name__ == "__main__": main()