IEP of Sequence from 1 to 561 Isoelectric Point = 5.0278 pH Bound Charge 1.00 108.00 1.00 1.50 107.99 0.99 2.00 107.98 0.98 2.50 107.93 0.93 3.00 107.80 0.80 3.50 107.56 0.56 4.00 107.28 0.28 4.50 107.10 0.10 5.00 107.00 0.00 5.50 106.91 -0.09 6.00 106.67 -0.33 6.50 105.94 -1.06 7.00 103.73 -3.27 7.50 97.29 -9.71 8.00 81.33 -25.67 8.50 53.56 -53.44 9.00 25.75 -81.25 9.50 9.75 -97.25 10.00 3.29 -103.71 10.50 1.06 -105.94 11.00 0.34 -106.66 11.50 0.11 -106.89 12.00 0.03 -106.97 12.50 0.01 -106.99 13.00 0.00 -107.00 13.50 0.00 -107.00 14.00 0.00 -107.00