| 1 | #!/usr/bin/python2.6 |
|---|
| 2 | |
|---|
| 3 | """ |
|---|
| 4 | Reads a list of intervals and an axt. Produces a new axt containing the |
|---|
| 5 | portions of the original that overlapped the intervals |
|---|
| 6 | |
|---|
| 7 | usage: %prog interval_file refindex [options] < axt_file |
|---|
| 8 | -m, --mincols=10: Minimum length (columns) required for alignment to be output |
|---|
| 9 | """ |
|---|
| 10 | |
|---|
| 11 | import psyco_full |
|---|
| 12 | |
|---|
| 13 | from bx.cookbook import doc_optparse |
|---|
| 14 | |
|---|
| 15 | import bx.align.axt |
|---|
| 16 | from bx import intervals |
|---|
| 17 | import sys |
|---|
| 18 | |
|---|
| 19 | |
|---|
| 20 | def __main__(): |
|---|
| 21 | |
|---|
| 22 | # Parse Command Line |
|---|
| 23 | |
|---|
| 24 | options, args = doc_optparse.parse( __doc__ ) |
|---|
| 25 | |
|---|
| 26 | try: |
|---|
| 27 | range_filename = args[ 0 ] |
|---|
| 28 | refindex = int( args[ 1 ] ) |
|---|
| 29 | if options.mincols: mincols = int( options.mincols ) |
|---|
| 30 | else: mincols = 10 |
|---|
| 31 | except: |
|---|
| 32 | doc_optparse.exit() |
|---|
| 33 | |
|---|
| 34 | # Load Intervals |
|---|
| 35 | |
|---|
| 36 | intersecter = intervals.Intersecter() |
|---|
| 37 | for line in file( range_filename ): |
|---|
| 38 | fields = line.split() |
|---|
| 39 | intersecter.add_interval( intervals.Interval( int( fields[0] ), int( fields[1] ) ) ) |
|---|
| 40 | |
|---|
| 41 | # Start axt on stdout |
|---|
| 42 | |
|---|
| 43 | out = bx.align.axt.Writer( sys.stdout ) |
|---|
| 44 | |
|---|
| 45 | # Iterate over input axt |
|---|
| 46 | |
|---|
| 47 | for axt in bx.align.axt.Reader( sys.stdin ): |
|---|
| 48 | ref_component = axt.components[ refindex ] |
|---|
| 49 | # Find overlap with reference component |
|---|
| 50 | intersections = intersecter.find( ref_component.start, ref_component.end ) |
|---|
| 51 | # Keep output axt ordered |
|---|
| 52 | intersections.sort() |
|---|
| 53 | # Write each intersecting block |
|---|
| 54 | for interval in intersections: |
|---|
| 55 | start = max( interval.start, ref_component.start ) |
|---|
| 56 | end = min( interval.end, ref_component.end ) |
|---|
| 57 | sliced = axt.slice_by_component( refindex, start, end ) |
|---|
| 58 | good = True |
|---|
| 59 | for c in sliced.components: |
|---|
| 60 | if c.size < 1: |
|---|
| 61 | good = False |
|---|
| 62 | if good and sliced.text_size > mincols: out.write( sliced ) |
|---|
| 63 | |
|---|
| 64 | # Close output axt |
|---|
| 65 | |
|---|
| 66 | out.close() |
|---|
| 67 | |
|---|
| 68 | if __name__ == "__main__": __main__() |
|---|