1 | <tool id="microbial_import1" name="Get Microbial Data">
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2 | <command interpreter="python">microbial_import.py $CDS,$tRNA,$rRNA,$sequence,$GeneMark,$GeneMarkHMM,$Glimmer3 $output ${GALAXY_DATA_INDEX_DIR}/microbial_data.loc</command>
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3 | <inputs>
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4 | <param name="kingdom" type="select" label="Select the Desired Kingdom">
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5 | <options from_file="microbial_data.loc" startswith="ORG">
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6 | <column name="name" index="3"/>
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7 | <column name="value" index="3"/>
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8 | <filter type="unique_value" name="unique" column="3"/>
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9 | </options>
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10 | </param>
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11 | <param name="org" type="select" label="Select the Desired Organism">
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12 | <options from_file="microbial_data.loc" startswith="ORG">
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13 | <column name="name" index="2"/>
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14 | <column name="value" index="1"/>
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15 | <filter type="param_value" ref="kingdom" name="kingdom" column="3"/>
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16 | <filter type="sort_by" column="2"/>
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17 | </options>
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18 | </param>
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19 | <param name="CDS" type="select" label="Select Desired Coding Sequences" display="checkboxes" multiple="True">
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20 | <options from_file="microbial_data.loc" startswith="DATA">
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21 | <column name="name" index="3"/>
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22 | <column name="value" index="1"/>
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23 | <column name="feature" index="4"/>
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24 | <filter type="param_value" ref="org" name="kingdom" column="2"/>
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25 | <filter type="static_value" name="feature" value="CDS" column="4"/>
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26 | </options>
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27 | </param>
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28 | <param name="tRNA" type="select" label="Select Desired tRNA" display="checkboxes" multiple="True">
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29 | <options from_file="microbial_data.loc" startswith="DATA">
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30 | <column name="name" index="3"/>
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31 | <column name="value" index="1"/>
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32 | <column name="feature" index="4"/>
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33 | <filter type="param_value" ref="org" name="kingdom" column="2"/>
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34 | <filter type="static_value" name="feature" value="tRNA" column="4"/>
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35 | </options>
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36 | </param>
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37 | <param name="rRNA" type="select" label="Select Desired rRNA" display="checkboxes" multiple="True">
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38 | <options from_file="microbial_data.loc" startswith="DATA">
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39 | <column name="name" index="3"/>
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40 | <column name="value" index="1"/>
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41 | <column name="feature" index="4"/>
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42 | <filter type="param_value" ref="org" name="kingdom" column="2"/>
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43 | <filter type="static_value" name="feature" value="rRNA" column="4"/>
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44 | </options>
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45 | </param>
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46 | <param name="sequence" type="select" label="Select Desired DNA Sequences" display="checkboxes" multiple="True">
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47 | <options from_file="microbial_data.loc" startswith="DATA">
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48 | <column name="name" index="3"/>
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49 | <column name="value" index="1"/>
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50 | <column name="feature" index="4"/>
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51 | <filter type="param_value" ref="org" name="kingdom" column="2"/>
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52 | <filter type="static_value" name="feature" value="sequence" column="4"/>
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53 | </options>
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54 | </param>
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55 | <param name="GeneMark" type="select" label="Select Desired GeneMark Annotations" display="checkboxes" multiple="True">
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56 | <options from_file="microbial_data.loc" startswith="DATA">
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57 | <column name="name" index="3"/>
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58 | <column name="value" index="1"/>
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59 | <column name="feature" index="4"/>
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60 | <filter type="param_value" ref="org" name="kingdom" column="2"/>
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61 | <filter type="static_value" name="feature" value="GeneMark" column="4"/>
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62 | </options>
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63 | </param>
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64 | <param name="GeneMarkHMM" type="select" label="Select Desired GeneMarkHMM Annotations" display="checkboxes" multiple="True">
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65 | <options from_file="microbial_data.loc" startswith="DATA">
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66 | <column name="name" index="3"/>
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67 | <column name="value" index="1"/>
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68 | <column name="feature" index="4"/>
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69 | <filter type="param_value" ref="org" name="kingdom" column="2"/>
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70 | <filter type="static_value" name="feature" value="GeneMarkHMM" column="4"/>
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71 | </options>
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72 | </param>
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73 | <param name="Glimmer3" type="select" label="Select Desired Glimmer3 Annotations" display="checkboxes" multiple="True">
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74 | <options from_file="microbial_data.loc" startswith="DATA">
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75 | <column name="name" index="3"/>
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76 | <column name="value" index="1"/>
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77 | <column name="feature" index="4"/>
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78 | <filter type="param_value" ref="org" name="kingdom" column="2"/>
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79 | <filter type="static_value" name="feature" value="Glimmer3" column="4"/>
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80 | </options>
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81 | </param>
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82 | </inputs>
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83 | <outputs>
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84 | <data format="bed" name="output"/>
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85 | </outputs>
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86 | <code file="microbial_import_code.py"/>
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87 | <help>
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88 |
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89 | This tool will allow you to obtain various genomic datasets for any completed Microbial Genome Project as listed at NCBI_.
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90 |
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91 | .. _NCBI: http://www.ncbi.nlm.nih.gov/genomes/lproks.cgi?view=1
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92 |
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93 | Current datasets available include
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94 | 1. CDS
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95 | 2. tRNA
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96 | 3. rRNA
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97 | 4. FASTA Sequences
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98 | 5. GeneMark Annotations
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99 | 6. GeneMarkHMM Annotations
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100 | 7. Glimmer3 Annotations
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101 |
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102 | -----
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103 |
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104 | Organisms in **bold** are available at the UCSC Browser.
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105 |
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106 | -----
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107 |
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108 | .. class:: infomark
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109 |
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110 | **Note:** Having trouble locating your organism? Click here_ for a list of available species and their location.
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111 |
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112 | .. _here: http://bitbucket.org/galaxy/galaxy-central/wiki/Microbes
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113 |
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114 | </help>
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115 | </tool>
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