1 | <tool id="EMBOSS: dan19" name="dan" version="5.0.0">
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2 | <description>Calculates DNA RNA/DNA melting temperature</description>
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3 | <requirements><requirement type="package" version="5.0.0">emboss</requirement></requirements>
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4 | <command interpreter="perl">emboss_single_outputfile_wrapper.pl dan -sequence $input1 -windowsize $window -goutfile $out_file1 -graph png -plot $plot1 -shiftincrement $shift -dnaconc $dnaconc
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5 | -saltconc $saltconc -product $product -formamide $formamide -mismatch $mismatch -prodlen $prodlen -thermo $thermo -temperature $temperature -rna $rna -outfile $out_file1 -auto</command>
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6 | <inputs>
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7 | <param format="data" name="input1" type="data">
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8 | <label>On query</label>
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9 | </param>
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10 | <param name="window" size="4" type="text" value="20">
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11 | <label>Window Size</label>
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12 | </param>
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13 | <param name="shift" size="4" type="text" value="1">
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14 | <label>Step size (shift increment)</label>
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15 | </param>
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16 | <param name="dnaconc" size="4" type="text" value="50.0">
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17 | <label>DNA Concentration (nM)</label>
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18 | </param>
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19 | <param name="saltconc" size="4" type="text" value="50.0">
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20 | <label>Salt concentration (mM)</label>
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21 | </param>
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22 | <param name="thermo" type="select">
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23 | <label>Output the DeltaG, DeltaH and DeltaS values</label>
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24 | <option value="yes">Yes</option>
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25 | <option value="no">No</option>
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26 | </param>
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27 | <param name="temperature" size="4" type="text" value="25 ">
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28 | <label>Temperature at which to calculate the DeltaG, DeltaH and DeltaS values</label>
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29 | </param>
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30 | <param name="rna" type="select">
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31 | <label>Sequence is RNA</label>
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32 | <option value="no">No</option>
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33 | <option value="yes">Yes</option>
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34 | </param>
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35 | <param name="product" type="select">
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36 | <label>Include percent formamide, percent of mismatches allowed and product length</label>
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37 | <option value="no">No</option>
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38 | <option value="yes">Yes</option>
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39 | </param>
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40 | <param name="formamide" size="4" type="text" value="0 ">
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41 | <label>Formamide concentration (nM)</label>
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42 | </param>
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43 | <param name="mismatch" size="4" type="text" value="0 ">
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44 | <label>Percent mismatch to be used in calculations</label>
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45 | </param>
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46 | <param name="prodlen" size="4" type="text" value="20">
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47 | <label>Product length to be used in calculations</label>
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48 | </param>
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49 | <param name="plot1" type="select">
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50 | <label>Create a graph</label>
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51 | <option value="no">No</option>
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52 | <option value="yes">Yes</option>
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53 | </param>
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54 | </inputs>
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55 | <outputs>
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56 | <data format="dan" name="out_file1" />
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57 | </outputs>
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58 | <!-- |
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59 | <tests> |
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60 | <test> |
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61 | <param name="input1" value="2.fasta"/> |
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62 | <param name="window" value="20"/> |
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63 | <param name="shift" value="1"/> |
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64 | <param name="dnaconc" value="50"/> |
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65 | <param name="saltconc" value="50"/> |
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66 | <param name="thermo" value="yes"/> |
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67 | <param name="temperature" value="25"/> |
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68 | <param name="rna" value="no"/> |
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69 | <param name="product" value="no"/> |
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70 | <param name="formamide" value="0"/> |
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71 | <param name="mismatch" value="0"/> |
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72 | <param name="prodlen" value="20"/> |
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73 | <param name="plot1" value="yes"/> |
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74 | <output name="out_file1" file="emboss_dan_out.png"/> |
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75 | </test> |
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76 | </tests>
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77 | -->
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78 | <code file="emboss_format_corrector.py" />
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79 | <help> |
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80 | You can view the original documentation here_. |
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81 | |
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82 | .. _here: http://emboss.sourceforge.net/apps/release/5.0/emboss/apps/dan.html |
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83 | </help> |
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84 | </tool> |
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