1 | <tool id="EMBOSS: digest23" name="digest" version="5.0.0">
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2 | <description>Protein proteolytic enzyme or reagent cleavage digest</description>
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3 | <requirements><requirement type="package" version="5.0.0">emboss</requirement></requirements>
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4 | <command>digest -seqall $input1 -outfile $out_file1 -menu $menu -unfavoured $unfavoured -overlap $overlap -allpartials $allpartials -rformat2 $out_format1 -auto</command>
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5 | <inputs>
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6 | <param format="data" name="input1" type="data">
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7 | <label>Sequence</label>
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8 | </param>
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9 | <param name="menu" type="select">
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10 | <label>Enzyme/Reagent</label>
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11 | <option value="1">Trypsin</option>
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12 | <option value="2">Lys-C</option>
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13 | <option value="3">Arg-C</option>
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14 | <option value="4">Asp-N</option>
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15 | <option value="5">V8-bicarb</option>
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16 | <option value="6">V8-phosph</option>
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17 | <option value="7">Chymotrypsin</option>
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18 | <option value="8">CNBr</option>
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19 | </param>
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20 | <param name="unfavoured" type="select">
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21 | <label>Trypsin will not normally cut after a K if it is followed by (e.g.) another K or a P. Specifying this shows those cuts, as well as the favoured ones.</label>
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22 | <option value="no">No</option>
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23 | <option value="yes">Yes</option>
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24 | </param>
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25 | <param name="overlap" type="select">
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26 | <label>Used for partial digestion. Shows all cuts from favoured cut sites plus 1..3, 2..4, 3..5 etc but not (e.g.) 2..5. Overlaps are therefore fragments with exactly one potential cut site
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27 | within it.</label>
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28 | <option value="no">No</option>
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29 | <option value="yes">Yes</option>
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30 | </param>
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31 | <param name="allpartials" type="select">
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32 | <label>As for overlap but fragments containing more than one potential cut site are included.</label>
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33 | <option value="no">No</option>
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34 | <option value="yes">Yes</option>
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35 | </param>
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36 | <param name="out_format1" type="select">
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37 | <label>Output Report File Format</label>
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38 | <option value="seqtable">SeqTable</option>
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39 | <option value="embl">EMBL</option>
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40 | <option value="genbank">GENBANK</option>
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41 | <option value="gff">GFF</option>
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42 | <option value="pir">PIR</option>
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43 | <option value="swiss">SwissProt</option>
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44 | <option value="dbmotif">DbMotif</option>
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45 | <option value="diffseq">Diffseq</option>
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46 | <option value="excel">Excel (tab delimited)</option>
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47 | <option value="feattable">FeatTable</option>
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48 | <option value="motif">Motif</option>
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49 | <option value="regions">Regions</option>
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50 | <option value="simple">SRS Simple</option>
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51 | <option value="srs">SRS</option>
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52 | <option value="table">Table</option>
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53 | <option value="tagseq">TagSeq</option>
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54 | </param>
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55 | </inputs>
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56 | <outputs>
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57 | <data format="digest" name="out_file1" />
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58 | </outputs>
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59 | <code file="emboss_format_corrector.py" />
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60 | <help> |
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61 | You can view the original documentation here_. |
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62 | |
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63 | .. _here: http://emboss.sourceforge.net/apps/release/5.0/emboss/apps/digest.html |
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64 | </help> |
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65 | </tool> |
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