root/galaxy-central/tools/fastx_toolkit/fastq_quality_boxplot.xml

<tool id="cshl_fastq_quality_boxplot" name="Draw quality score boxplot">
        <description></description>
        <requirements><requirement type="package">fastx_toolkit</requirement></requirements>
        
        <command>fastq_quality_boxplot_graph.sh -t '$input.name' -i $input -o $output</command>
        
        <inputs>
                <param format="txt" name="input" type="data" label="Statistics report file"  help="output of 'FASTQ Statistics' tool" />
        </inputs>

        <outputs>
                <data format="png" name="output" metadata_source="input" />
        </outputs>
<help>

**What it does**

Creates a boxplot graph for the quality scores in the library.

.. class:: infomark

**TIP:** Use the **FASTQ Statistics** tool to generate the report file needed for this tool.

-----

**Output Examples**

* Black horizontal lines are medians
* Rectangular red boxes show the Inter-quartile Range (IQR) (top value is Q3, bottom value is Q1)
* Whiskers show outlier at max. 1.5*IQR


An excellent quality library (median quality is 40 for almost all 36 cycles):

.. image:: ../static/fastx_icons/fastq_quality_boxplot_1.png


A relatively good quality library (median quality degrades towards later cycles):

.. image:: ../static/fastx_icons/fastq_quality_boxplot_2.png

A low quality library (median drops quickly):

.. image:: ../static/fastx_icons/fastq_quality_boxplot_3.png

------

This tool is based on `FASTX-toolkit`__ by Assaf Gordon.

 .. __: http://hannonlab.cshl.edu/fastx_toolkit/



</help>
</tool>
<!-- FASTQ-Quality-Boxplot is part of the FASTX-toolkit, by A.Gordon (gordon@cshl.edu) -->