root/galaxy-central/tools/fastx_toolkit/fastq_quality_converter.xml

<tool id="cshl_fastq_quality_converter" name="Quality format converter">
        <description>(ASCII-Numeric)</description>
        <requirements><requirement type="package">fastx_toolkit</requirement></requirements>
        <command>zcat -f $input | fastq_quality_converter $QUAL_FORMAT -o $output -Q $offset</command>
        <inputs>
                <param format="fastq" name="input" type="data" label="Library to convert" />

                <param name="QUAL_FORMAT" type="select" label="Desired output format">
                        <option value="-a">ASCII (letters) quality scores</option>
                        <option value="-n">Numeric quality scores</option>
                </param>
                
        <param name="offset" type="select" label="FASTQ ASCII offset">
            <option value="33">33</option>
            <option selected="true" value="64">64</option>
        </param>        
    </inputs>

        <tests>
                <test>
                        <!-- ASCII to NUMERIC -->
                        <param name="input" value="fastq_qual_conv1.fastq" />
                        <param name="QUAL_FORMAT" value="Numeric quality scores" />
                        <param name="offset" value="64" />
                        <output name="output" file="fastq_qual_conv1.out" />
                </test>
                <test>
                        <!-- ASCII to ASCII (basically, a no-op, but it should still produce a valid output -->
                        <param name="input" value="fastq_qual_conv1.fastq" />
                        <param name="QUAL_FORMAT" value="ASCII (letters) quality scores" />
                        <param name="offset" value="64" />
                        <output name="output" file="fastq_qual_conv1a.out" />
                </test>
                <test>
                        <!-- NUMERIC to ASCII -->
                        <param name="input" value="fastq_qual_conv2.fastq" />
                        <param name="QUAL_FORMAT" value="ASCII (letters) quality scores" />
                        <param name="offset" value="64" />
                        <output name="output" file="fastq_qual_conv2.out" />
                </test>
                <test>
                        <!-- NUMERIC to NUMERIC (basically, a no-op, but it should still produce a valid output -->
                        <param name="input" value="fastq_qual_conv2.fastq" />
                        <param name="QUAL_FORMAT" value="Numeric quality scores" />
                        <param name="offset" value="64" />
                        <output name="output" file="fastq_qual_conv2n.out" />
                </test>
        </tests>

        <outputs>
                <data format="fastq" name="output" metadata_source="input" />
        </outputs>
<help>

**What it does**

Converts a Solexa FASTQ file to/from numeric or ASCII quality format.

.. class:: warningmark 

Re-scaling is **not** performed. (e.g. conversion from Phred scale to Solexa scale).


-----

FASTQ with Numeric quality scores::

    @CSHL__2_FC042AGWWWXX:8:1:120:202
    ACGATAGATCGGAAGAGCTAGTATGCCGTTTTCTGC
    +CSHL__2_FC042AGWWWXX:8:1:120:202
    40 40 40 40 20 40 40 40 40 6 40 40 28 40 40 25 40 20 40 -1 30 40 14 27 40 8 1 3 7 -1 11 10 -1 21 10 8
    @CSHL__2_FC042AGWWWXX:8:1:103:1185
    ATCACGATAGATCGGCAGAGCTCGTTTACCGTCTTC
    +CSHL__2_FC042AGWWWXX:8:1:103:1185
    40 40 40 40 40 35 33 31 40 40 40 32 30 22 40 -0 9 22 17 14 8 36 15 34 22 12 23 3 10 -0 8 2 4 25 30 2


FASTQ with ASCII quality scores::

    @CSHL__2_FC042AGWWWXX:8:1:120:202
    ACGATAGATCGGAAGAGCTAGTATGCCGTTTTCTGC
    +CSHL__2_FC042AGWWWXX:8:1:120:202
    hhhhThhhhFhh\hhYhTh?^hN[hHACG?KJ?UJH
    @CSHL__2_FC042AGWWWXX:8:1:103:1185
    ATCACGATAGATCGGCAGAGCTCGTTTACCGTCTTC
    +CSHL__2_FC042AGWWWXX:8:1:103:1185
    hhhhhca_hhh`^Vh@IVQNHdObVLWCJ@HBDY^B

------

This tool is based on `FASTX-toolkit`__ by Assaf Gordon.

 .. __: http://hannonlab.cshl.edu/fastx_toolkit/
 
</help>
</tool>
<!-- FASTQ-Quality-Converter is part of the FASTX-toolkit, by A.Gordon (gordon@cshl.edu) -->