1 | <tool id="cshl_fastq_to_fasta" name="FASTQ to FASTA"> |
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2 | <description>converter</description> |
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3 | <requirements><requirement type="package">fastx_toolkit</requirement></requirements>
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4 | <command>gunzip -cf $input | fastq_to_fasta $SKIPN $RENAMESEQ -o $output -v </command> |
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5 | |
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6 | <inputs> |
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7 | <param format="fastq" name="input" type="data" label="FASTQ Library to convert" /> |
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8 | |
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9 | <param name="SKIPN" type="select" label="Discard sequences with unknown (N) bases "> |
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10 | <option value="">yes</option> |
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11 | <option value="-n">no</option> |
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12 | </param> |
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13 | |
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14 | <param name="RENAMESEQ" type="select" label="Rename sequence names in output file (reduces file size)"> |
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15 | <option value="-r">yes</option> |
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16 | <option value="">no</option> |
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17 | </param> |
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18 | |
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19 | </inputs> |
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20 | |
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21 | <tests> |
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22 | <test> |
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23 | <!-- FASTQ-To-FASTA, keep N, don't rename --> |
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24 | <param name="input" value="fastq_to_fasta1.fastq" /> |
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25 | <param name="SKIPN" value=""/> |
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26 | <param name="RENAMESEQ" value=""/> |
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27 | <output name="output" file="fastq_to_fasta1a.out" /> |
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28 | </test> |
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29 | <test> |
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30 | <!-- FASTQ-To-FASTA, discard N, rename --> |
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31 | <param name="input" value="fastq_to_fasta1.fastq" /> |
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32 | <param name="SKIPN" value="no"/> |
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33 | <param name="RENAMESEQ" value="yes"/> |
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34 | <output name="output" file="fastq_to_fasta1b.out" /> |
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35 | </test> |
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36 | </tests> |
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37 | |
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38 | <outputs> |
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39 | <data format="fasta" name="output" metadata_source="input" /> |
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40 | </outputs> |
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41 | |
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42 | <help> |
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43 | |
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44 | **What it does** |
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45 | |
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46 | This tool converts data from Solexa format to FASTA format (scroll down for format description). |
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47 | |
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48 | -------- |
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49 | |
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50 | **Example** |
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51 | |
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52 | The following data in Solexa-FASTQ format:: |
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53 | |
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54 | @CSHL_4_FC042GAMMII_2_1_517_596 |
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55 | GGTCAATGATGAGTTGGCACTGTAGGCACCATCAAT |
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56 | +CSHL_4_FC042GAMMII_2_1_517_596 |
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57 | 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 40 14 40 40 40 40 40 36 40 13 14 24 24 9 24 9 40 10 10 15 40 |
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58 | |
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59 | Will be converted to FASTA (with 'rename sequence names' = NO):: |
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60 | |
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61 | >CSHL_4_FC042GAMMII_2_1_517_596 |
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62 | GGTCAATGATGAGTTGGCACTGTAGGCACCATCAAT |
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63 | |
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64 | Will be converted to FASTA (with 'rename sequence names' = YES):: |
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65 | |
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66 | >1 |
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67 | GGTCAATGATGAGTTGGCACTGTAGGCACCATCAAT |
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68 | |
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69 | ------ |
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70 | |
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71 | This tool is based on `FASTX-toolkit`__ by Assaf Gordon. |
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72 | |
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73 | .. __: http://hannonlab.cshl.edu/fastx_toolkit/ |
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74 | </help> |
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75 | </tool> |
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76 | <!-- FASTQ-to-FASTA is part of the FASTX-toolkit, by A.Gordon (gordon@cshl.edu) --> |
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