1 | <tool id="cshl_fastx_renamer" name="Rename sequences" version="0.0.11" > |
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2 | <description></description> |
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3 | <requirements><requirement type="package">fastx_toolkit</requirement></requirements>
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4 | <command>zcat -f $input | fastx_renamer -n $TYPE -o $output -v </command> |
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5 | |
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6 | <inputs> |
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7 | <param format="fastqsolexa,fasta,fastqsanger" name="input" type="data" label="FASTQ/A Library to rename" /> |
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8 | |
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9 | <param name="TYPE" type="select" label="Rename sequence identifiers to"> |
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10 | <option value="SEQ">Nucleotides sequence</option> |
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11 | <option value="COUNT">Numeric Counter</option> |
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12 | </param> |
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13 | </inputs> |
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14 | |
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15 | <outputs> |
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16 | <data format="input" name="output" metadata_source="input" /> |
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17 | </outputs> |
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18 | |
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19 | <help> |
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20 | |
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21 | **What it does** |
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22 | |
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23 | This tool renames the sequence identifiers in a FASTQ/A file. |
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24 | |
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25 | .. class:: infomark |
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26 | |
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27 | Use this tool at the beginning of your workflow, as a way to keep the original sequence (before trimming, clipping, barcode-removal, etc). |
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28 | |
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29 | -------- |
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30 | |
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31 | **Example** |
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32 | |
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33 | The following Solexa-FASTQ file:: |
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34 | |
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35 | @CSHL_4_FC042GAMMII_2_1_517_596 |
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36 | GGTCAATGATGAGTTGGCACTGTAGGCACCATCAAT |
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37 | +CSHL_4_FC042GAMMII_2_1_517_596 |
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38 | 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 40 14 40 40 40 40 40 36 40 13 14 24 24 9 24 9 40 10 10 15 40 |
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39 | |
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40 | Renamed to **nucleotides sequence**:: |
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41 | |
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42 | @GGTCAATGATGAGTTGGCACTGTAGGCACCATCAAT |
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43 | GGTCAATGATGAGTTGGCACTGTAGGCACCATCAAT |
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44 | +GGTCAATGATGAGTTGGCACTGTAGGCACCATCAAT |
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45 | 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 40 14 40 40 40 40 40 36 40 13 14 24 24 9 24 9 40 10 10 15 40 |
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46 | |
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47 | Renamed to **numeric counter**:: |
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48 | |
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49 | @1 |
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50 | GGTCAATGATGAGTTGGCACTGTAGGCACCATCAAT |
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51 | +1 |
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52 | 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 40 14 40 40 40 40 40 36 40 13 14 24 24 9 24 9 40 10 10 15 40 |
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53 | |
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54 | ------ |
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55 | |
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56 | This tool is based on `FASTX-toolkit`__ by Assaf Gordon. |
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57 | |
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58 | .. __: http://hannonlab.cshl.edu/fastx_toolkit/ |
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59 | </help> |
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60 | </tool> |
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61 | <!-- FASTQ-to-FASTA is part of the FASTX-toolkit, by A.Gordon (gordon@cshl.edu) --> |
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