root/galaxy-central/tools/fastx_toolkit/fastx_trimmer.xml @ 3

<tool id="cshl_fastx_trimmer" name="Trim sequences">
        <description></description>
        <requirements><requirement type="package">fastx_toolkit</requirement></requirements>
        <command>zcat -f '$input' | fastx_trimmer -v -f $first -l $last -o $output</command>

        <inputs>
                <param format="fasta,fastqsanger" name="input" type="data" label="Library to clip" />

                <param name="first" size="4" type="integer" value="1">
                        <label>First base to keep</label>
                </param>

                <param name="last" size="4" type="integer" value="21">
                        <label>Last base to keep</label>
                </param>
        </inputs>

        <tests>
                <test>
                        <!-- Trim a FASTA file - remove first four bases (e.g. a barcode) -->
                        <param name="input" value="fastx_trimmer1.fasta" />
                        <param name="first" value="5"/>
                        <param name="last" value="36"/>
                        <output name="output" file="fastx_trimmer1.out" />
                </test>
                <test>
                        <!-- Trim a FASTQ file - remove last 9 bases (e.g. keep only miRNA length sequences) -->
                        <param name="input" value="fastx_trimmer2.fastq" />
                        <param name="first" value="1"/>
                        <param name="last" value="27"/>
                        <output name="output" file="fastx_trimmer2.out" />
                </test>
        </tests>

        <outputs>
                <data format="input" name="output" metadata_source="input" />
        </outputs>
        <help>
**What it does**

This tool trims (cut bases from) sequences in a FASTA/Q file.
  
--------

**Example**

Input Fasta file (with 36 bases in each sequences)::

    >1-1
    TATGGTCAGAAACCATATGCAGAGCCTGTAGGCACC
    >2-1
    CAGCGAGGCTTTAATGCCATTTGGCTGTAGGCACCA
    

Trimming with First=1 and Last=21, we get a FASTA file with 21 bases in each sequences (starting from the first base)::

    >1-1
    TATGGTCAGAAACCATATGCA
    >2-1
    CAGCGAGGCTTTAATGCCATT

Trimming with First=6 and Last=10, will generate a FASTA file with 5 bases (bases 6,7,8,9,10) in each sequences::

    >1-1
    TCAGA
    >2-1
    AGGCT
    
    ------

This tool is based on `FASTX-toolkit`__ by Assaf Gordon.

 .. __: http://hannonlab.cshl.edu/fastx_toolkit/
    
</help>
</tool>
<!-- FASTX-Trimmer is part of the FASTX-toolkit, by A.Gordon (gordon@cshl.edu) -->