1 | #!/usr/bin/env python |
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2 | |
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3 | """ |
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4 | Read a maf and output a single block fasta file, concatenating blocks |
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5 | |
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6 | usage %prog species1,species2 maf_file out_file |
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7 | """ |
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8 | #Dan Blankenberg |
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9 | import sys |
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10 | from galaxy import eggs |
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11 | import pkg_resources; pkg_resources.require( "bx-python" ) |
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12 | from bx.align import maf |
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13 | from galaxy.tools.util import maf_utilities |
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14 | |
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15 | assert sys.version_info[:2] >= ( 2, 4 ) |
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16 | |
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17 | def __main__(): |
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18 | try: |
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19 | species = maf_utilities.parse_species_option( sys.argv[1] ) |
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20 | except Exception, e: |
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21 | maf_utilities.tool_fail( "Error determining species value: %s" % e ) |
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22 | try: |
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23 | input_filename = sys.argv[2] |
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24 | except Exception, e: |
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25 | maf_utilities.tool_fail( "Error reading MAF filename: %s" % e ) |
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26 | try: |
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27 | file_out = open( sys.argv[3], 'w' ) |
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28 | except Exception, e: |
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29 | maf_utilities.tool_fail( "Error opening file for output: %s" % e ) |
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30 | |
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31 | if species: |
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32 | print "Restricted to species: %s" % ', '.join( species ) |
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33 | else: |
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34 | print "Not restricted to species." |
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35 | |
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36 | if not species: |
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37 | try: |
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38 | species = maf_utilities.get_species_in_maf( input_filename ) |
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39 | except Exception, e: |
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40 | maf_utilities.tool_fail( "Error determining species in input MAF: %s" % e ) |
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41 | |
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42 | for spec in species: |
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43 | file_out.write( ">" + spec + "\n" ) |
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44 | try: |
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45 | for start_block in maf.Reader( open( input_filename, 'r' ) ): |
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46 | for block in maf_utilities.iter_blocks_split_by_species( start_block ): |
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47 | block.remove_all_gap_columns() #remove extra gaps |
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48 | component = block.get_component_by_src_start( spec ) #blocks only have one occurrence of a particular species, so this is safe |
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49 | if component: |
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50 | file_out.write( component.text ) |
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51 | else: |
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52 | file_out.write( "-" * block.text_size ) |
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53 | except Exception, e: |
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54 | maf_utilities.tool_fail( "Your MAF file appears to be malformed: %s" % e ) |
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55 | file_out.write( "\n" ) |
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56 | file_out.close() |
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57 | |
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58 | |
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59 | if __name__ == "__main__": __main__() |
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