1 | #!/usr/bin/env python |
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2 | """ |
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3 | Concatenate two bed files. The concatenated files are returned in the |
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4 | same format as the first. If --sameformat is specified, then all |
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5 | columns will be treated as the same, and all fields will be saved, |
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6 | although the output will be trimmed to match the primary input. In |
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7 | addition, if --sameformat is specified, missing fields will be padded |
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8 | with a period(.). |
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9 | |
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10 | usage: %prog in_file_1 in_file_2 out_file |
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11 | -1, --cols1=N,N,N,N: Columns for chrom, start, end, strand in first file |
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12 | -2, --cols2=N,N,N,N: Columns for chrom, start, end, strand in second file |
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13 | -s, --sameformat: All files are precisely the same format. |
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14 | """ |
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15 | from galaxy import eggs |
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16 | import pkg_resources |
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17 | pkg_resources.require( "bx-python" ) |
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18 | import sys, traceback, fileinput |
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19 | from warnings import warn |
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20 | from bx.intervals import * |
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21 | from bx.intervals.io import * |
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22 | from bx.intervals.operations.concat import * |
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23 | from bx.cookbook import doc_optparse |
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24 | from galaxy.tools.util.galaxyops import * |
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25 | |
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26 | assert sys.version_info[:2] >= ( 2, 4 ) |
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27 | |
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28 | def main(): |
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29 | sameformat=False |
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30 | upstream_pad = 0 |
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31 | downstream_pad = 0 |
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32 | |
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33 | options, args = doc_optparse.parse( __doc__ ) |
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34 | try: |
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35 | chr_col_1, start_col_1, end_col_1, strand_col_1 = parse_cols_arg( options.cols1 ) |
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36 | chr_col_2, start_col_2, end_col_2, strand_col_2 = parse_cols_arg( options.cols2 ) |
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37 | if options.sameformat: sameformat = True |
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38 | in_file_1, in_file_2, out_fname = args |
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39 | except: |
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40 | doc_optparse.exception() |
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41 | |
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42 | g1 = NiceReaderWrapper( fileinput.FileInput( in_file_1 ), |
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43 | chrom_col=chr_col_1, |
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44 | start_col=start_col_1, |
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45 | end_col=end_col_1, |
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46 | fix_strand=True ) |
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47 | |
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48 | g2 = NiceReaderWrapper( fileinput.FileInput( in_file_2 ), |
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49 | chrom_col=chr_col_2, |
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50 | start_col=start_col_2, |
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51 | end_col=end_col_2, |
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52 | strand_col=strand_col_2, |
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53 | fix_strand=True ) |
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54 | |
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55 | if strand_col_1 >= 0: |
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56 | g1.strand_col = strand_col_1 |
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57 | |
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58 | out_file = open( out_fname, "w" ) |
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59 | |
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60 | try: |
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61 | for line in concat( [g1, g2], sameformat=sameformat ): |
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62 | if type( line ) is GenomicInterval: |
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63 | out_file.write( "%s\n" % "\t".join( line.fields ) ) |
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64 | else: |
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65 | out_file.write( "%s\n" % line ) |
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66 | except ParseError, exc: |
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67 | out_file.close() |
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68 | fail( "Invalid file format: %s" % str( exc ) ) |
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69 | |
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70 | out_file.close() |
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71 | |
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72 | if g1.skipped > 0: |
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73 | print skipped( g1, filedesc=" of 1st dataset" ) |
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74 | if g2.skipped > 0: |
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75 | print skipped( g2, filedesc=" of 2nd dataset" ) |
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76 | |
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77 | if __name__ == "__main__": |
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78 | main() |
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