1 | <tool id="winSplitter" name="Make windows"> |
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2 | <description></description> |
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3 | <command interpreter="python">windowSplitter.py $input $size $out_file1 ${wintype.choice} ${wintype.offset} -l ${input.metadata.chromCol},${input.metadata.startCol},${input.metadata.endCol},${input.metadata.strandCol}</command> |
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4 | <inputs> |
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5 | <!--<param label="Genome" name="dbkey" type="genomebuild"/>--> |
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6 | <param format="interval" name="input" type="data" label="Select data"/> |
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7 | <param name="size" size="10" type="integer" value="500" label="Window size"/> |
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8 | <conditional name="wintype"> |
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9 | <param name="choice" type="select" label="Make sliding windows?"> |
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10 | <option value="0" selected="true">No</option> |
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11 | <option value="1">Yes</option> |
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12 | </param> |
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13 | <when value="0"> |
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14 | <param name="offset" type="hidden" value="0" /> |
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15 | </when> |
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16 | <when value="1"> |
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17 | <param name="offset" size="10" type="integer" value="10" label="Offset size"/> |
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18 | </when> |
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19 | </conditional> |
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20 | </inputs> |
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21 | <outputs> |
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22 | <data format="interval" name="out_file1" metadata_source="input"/> |
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23 | </outputs> |
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24 | <tests> |
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25 | <test> |
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26 | <param name="input" value="4.bed"/> |
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27 | <param name="size" value="5000"/> |
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28 | <param name="choice" value="1"/> |
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29 | <param name="offset" value="4000"/> |
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30 | <output name="out_file1" file="4_windows.bed"/> |
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31 | </test> |
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32 | </tests> |
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33 | <help> |
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34 | |
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35 | .. class:: infomark |
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36 | |
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37 | **What it does** |
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38 | |
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39 | This tool splits the intervals in the input file into smaller intervals based on the specified window-size and window type. |
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40 | |
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41 | ----- |
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42 | |
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43 | .. class:: warningmark |
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44 | |
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45 | **Note** |
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46 | |
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47 | The positions at the end of the input interval which do not fit into the last window or a new window of required size, will be omitted from the output. |
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48 | |
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49 | ----- |
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50 | |
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51 | .. class:: infomark |
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52 | |
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53 | **About formats** |
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54 | |
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55 | **BED format** Browser Extensible Data format was designed at UCSC for displaying data tracks in the Genome Browser. It has three required fields and several additional optional ones: |
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56 | |
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57 | The first three BED fields (required) are:: |
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58 | |
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59 | 1. chrom - The name of the chromosome (e.g. chr1, chrY_random). |
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60 | 2. chromStart - The starting position in the chromosome. (The first base in a chromosome is numbered 0.) |
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61 | 3. chromEnd - The ending position in the chromosome, plus 1 (i.e., a half-open interval). |
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62 | |
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63 | The additional BED fields (optional) are:: |
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64 | |
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65 | 4. name - The name of the BED line. |
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66 | 5. score - A score between 0 and 1000. |
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67 | 6. strand - Defines the strand - either '+' or '-'. |
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68 | 7. thickStart - The starting position where the feature is drawn thickly at the Genome Browser. |
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69 | 8. thickEnd - The ending position where the feature is drawn thickly at the Genome Browser. |
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70 | 9. reserved - This should always be set to zero. |
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71 | 10. blockCount - The number of blocks (exons) in the BED line. |
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72 | 11. blockSizes - A comma-separated list of the block sizes. The number of items in this list should correspond to blockCount. |
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73 | 12. blockStarts - A comma-separated list of block starts. All of the blockStart positions should be calculated relative to chromStart. The number of items in this list should correspond to blockCount. |
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74 | 13. expCount - The number of experiments. |
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75 | 14. expIds - A comma-separated list of experiment ids. The number of items in this list should correspond to expCount. |
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76 | 15. expScores - A comma-separated list of experiment scores. All of the expScores should be relative to expIds. The number of items in this list should correspond to expCount. |
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77 | |
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78 | ----- |
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79 | |
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80 | **Example** |
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81 | |
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82 | - For the following query:: |
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83 | |
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84 | chr22 1000 4700 NM_174568 0 + |
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85 | |
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86 | - running this tool with **Window size as 1000**, will return:: |
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87 | |
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88 | chr22 1000 2000 NM_174568 0 + |
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89 | chr22 2000 3000 NM_174568 0 + |
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90 | chr22 3000 4000 NM_174568 0 + |
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91 | |
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92 | - running this tool to make **Sliding windows** of **size 1000** and **offset 500**, will return:: |
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93 | |
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94 | chr22 1000 2000 NM_174568 0 + |
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95 | chr22 1500 2500 NM_174568 0 + |
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96 | chr22 2000 3000 NM_174568 0 + |
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97 | chr22 2500 3500 NM_174568 0 + |
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98 | chr22 3000 4000 NM_174568 0 + |
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99 | chr22 3500 4500 NM_174568 0 + |
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100 | |
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101 | </help> |
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102 | |
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103 | |
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104 | </tool> |
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