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11 | <div class="document"> |
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12 | <h4>Output from rgEigPCA.py run at 19/05/2010 15:14:48<br/> |
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13 | </h4> |
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14 | newfilepath=/opt/galaxy/test-data/rgtestouts/rgEigPCA, rexe=R(click on the image below to see a much higher quality PDF version)<table border="0" cellpadding="10" cellspacing="10"><tr><td> |
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15 | <a href="rgEigPCAtest1_PCAPlot.pdf"><img src="rgEigPCAtest1_PCAPlot.pdf.png" alt="Samples plotted in first 2 eigenvector space" hspace="10" align="left" /></a></td></tr></table><br/> |
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16 | <div class="document">All Files:<ol><li><a href="Rplots.pdf">Rplots.pdf (813 B)</a></li> |
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17 | <li><a href="rgEigPCAtest1.R">rgEigPCAtest1.R (1.6 KB)</a></li> |
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18 | <li><a href="rgEigPCAtest1.html">rgEigPCAtest1.html </a></li> |
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19 | <li><a href="rgEigPCAtest1.txt">rgEigPCAtest1.txt (3.3 KB)</a></li> |
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20 | <li><a href="rgEigPCAtest1_PCAPlot.pdf">rgEigPCAtest1_PCAPlot.pdf (7.9 KB)</a></li> |
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21 | <li><a href="rgEigPCAtest1_PCAPlot.pdf.png">rgEigPCAtest1_PCAPlot.pdf.png (27.1 KB)</a></li> |
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22 | <li><a href="rgEigPCAtest1_eigensoftplot.pdf.pdf">rgEigPCAtest1_eigensoftplot.pdf.pdf (2.1 KB)</a></li> |
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23 | <li><a href="rgEigPCAtest1_eigensoftplot.pdf.ps">rgEigPCAtest1_eigensoftplot.pdf.ps (13.6 KB)</a></li> |
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24 | <li><a href="rgEigPCAtest1_eigensoftplot.pdf.xtxt">rgEigPCAtest1_eigensoftplot.pdf.xtxt (257 B)</a></li> |
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25 | <li><a href="rgEigPCAtest1_eval.xls">rgEigPCAtest1_eval.xls (507 B)</a></li> |
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26 | <li><a href="rgEigPCAtest1_log.txt">rgEigPCAtest1_log.txt (6.0 KB)</a></li> |
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27 | <li><a href="rgEigPCAtest1_pca.xls">rgEigPCAtest1_pca.xls (1.3 KB)</a></li> |
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28 | <li><a href="rgEigPCAtest1_pca.xls.evec">rgEigPCAtest1_pca.xls.evec (3.3 KB)</a></li> |
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29 | <li><a href="rgEigPCAtest1_pca.xls.par">rgEigPCAtest1_pca.xls.par (311 B)</a></li> |
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30 | </ol></div><div class="document">Log rgEigPCAtest1_log.txt contents follow below<p/><pre>parameter file: rgEigPCAtest1_pca.xls.par |
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31 | ### THE INPUT PARAMETERS |
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32 | ##PARAMETER NAME: VALUE |
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33 | genotypename: /opt/galaxy/test-data/tinywga.bed |
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34 | snpname: /opt/galaxy/test-data/tinywga.bim |
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35 | indivname: /opt/galaxy/test-data/tinywga.fam |
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36 | evecoutname: rgEigPCAtest1_pca.xls.evec |
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37 | evaloutname: rgEigPCAtest1_eval.xls |
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38 | altnormstyle: NO |
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39 | numoutevec: 4 |
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40 | numoutlieriter: 2 |
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41 | numoutlierevec: 2 |
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42 | outliersigmathresh: 2 |
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43 | qtmode: 0 |
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44 | ## smartpca version: 8000 |
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45 | norm used |
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46 | |
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47 | genetic distance set from physical distance |
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48 | genotype file processed |
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49 | number of samples used: 40 number of snps used: 25 |
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50 | REMOVED outlier 1334:2 iter 1 evec 1 sigmage -2.329 |
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51 | number of samples after outlier removal: 39 |
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52 | |
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53 | ## Tracy-Widom statistics: rows: 39 cols: 24 |
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54 | #N eigenvalue difference twstat p-value effect. n |
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55 | 1 11.481974 NA -0.712 0.332198 8.854 |
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56 | 2 7.739771 -3.742203 -1.302 0.510496 8.388 |
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57 | 3 7.254586 -0.485185 -0.171 0.201072 7.095 |
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58 | 4 4.270950 -2.983636 -0.191 0.205302 8.005 |
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59 | 5 2.538320 -1.732630 -0.341 0.238117 9.118 |
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60 | 6 1.667964 -0.870356 -0.231 0.21376 9.910 |
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61 | 7 1.043403 -0.624560 -0.269 0.222042 11.590 |
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62 | 8 0.653401 -0.390003 NA NA NA |
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63 | 9 0.391044 -0.262357 NA NA NA |
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64 | 10 0.338164 -0.052880 NA NA NA |
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65 | 11 0.263384 -0.074780 NA NA NA |
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66 | 12 0.150750 -0.112634 NA NA NA |
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67 | 13 0.085580 -0.065170 NA NA NA |
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68 | 14 0.065301 -0.020279 NA NA NA |
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69 | 15 0.048797 -0.016504 NA NA NA |
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70 | 16 0.006611 -0.042186 NA NA NA |
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71 | kurtosis snps indivs |
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72 | eigenvector 1 1.926 2.122 |
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73 | eigenvector 2 2.599 2.148 |
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74 | eigenvector 3 2.593 2.873 |
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75 | eigenvector 4 3.165 2.831 |
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76 | population: 0 Case 9 |
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77 | population: 1 Control 30 |
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78 | |
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79 | ## Average divergence between populations: |
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80 | Case Control popsize |
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81 | Case 1.028 0.954 9 |
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82 | Control 0.954 0.972 30 |
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83 | |
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84 | |
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85 | number of blocks for moving block jackknife: 1 |
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86 | fst *1000: |
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87 | C C |
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88 | C 0 0 |
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89 | C 0 0 |
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90 | |
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91 | s.dev * 1000000: |
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92 | C C |
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93 | C 0 0 |
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94 | C 0 0 |
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95 | |
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96 | ## Anova statistics for population differences along each eigenvector: |
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97 | p-value |
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98 | eigenvector_1_Case_Control_ 0.841067 |
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99 | eigenvector_2_Case_Control_ 0.758376 |
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100 | eigenvector_3_Case_Control_ 0.238793 |
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101 | eigenvector_4_Case_Control_ 0.678458 |
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102 | |
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103 | ## Statistical significance of differences beween populations: |
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104 | pop1 pop2 chisq p-value |pop1| |pop2| |
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105 | popdifference: Case Control 1.810 0.77061 9 30 |
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106 | |
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107 | eigbestsnp 1 rs762601 22 21898858 1.674 |
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108 | eigbestsnp 1 rs2156921 22 21899063 1.674 |
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109 | eigbestsnp 1 rs4822375 22 21905642 1.674 |
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110 | eigbestsnp 1 rs5751611 22 21896019 1.635 |
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111 | eigbestsnp 1 rs4820537 22 21794810 1.542 |
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112 | eigbestsnp 1 rs3788347 22 21797804 1.413 |
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113 | eigbestsnp 1 rs2267000 22 21785366 1.151 |
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114 | eigbestsnp 1 rs4820539 22 21807970 1.141 |
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115 | eigbestsnp 1 rs5751592 22 21827674 0.890 |
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116 | eigbestsnp 1 rs2283804 22 21820335 0.815 |
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117 | eigbestsnp 1 rs2267006 22 21820990 0.815 |
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118 | eigbestsnp 2 rs5759608 22 21832708 2.197 |
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119 | eigbestsnp 2 rs5759612 22 21833170 2.197 |
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120 | eigbestsnp 2 rs2283804 22 21820335 1.643 |
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121 | eigbestsnp 2 rs2267006 22 21820990 1.643 |
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122 | eigbestsnp 2 rs2283802 22 21784722 1.283 |
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123 | eigbestsnp 2 rs2267009 22 21860168 1.283 |
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124 | eigbestsnp 2 rs2071436 22 21871488 1.283 |
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125 | eigbestsnp 2 rs756632 22 21799918 0.942 |
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126 | eigbestsnp 2 rs16997606 22 21794754 0.715 |
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127 | eigbestsnp 2 rs6003566 22 21889806 0.645 |
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128 | eigbestsnp 2 rs762601 22 21898858 0.545 |
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129 | eigbestsnp 3 rs16997606 22 21794754 1.770 |
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130 | eigbestsnp 3 rs6003566 22 21889806 1.725 |
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131 | eigbestsnp 3 rs3788347 22 21797804 1.452 |
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132 | eigbestsnp 3 rs2267000 22 21785366 1.386 |
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133 | eigbestsnp 3 rs2283802 22 21784722 1.247 |
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134 | eigbestsnp 3 rs2267009 22 21860168 1.247 |
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135 | eigbestsnp 3 rs2071436 22 21871488 1.247 |
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136 | eigbestsnp 3 rs4820537 22 21794810 1.168 |
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137 | eigbestsnp 3 rs762601 22 21898858 1.153 |
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138 | eigbestsnp 3 rs2156921 22 21899063 1.153 |
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139 | eigbestsnp 3 rs4822375 22 21905642 1.153 |
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140 | eigbestsnp 4 rs756632 22 21799918 2.146 |
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141 | eigbestsnp 4 rs4820539 22 21807970 1.938 |
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142 | eigbestsnp 4 rs2267013 22 21875879 1.917 |
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143 | eigbestsnp 4 rs2256725 22 21892891 1.917 |
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144 | eigbestsnp 4 rs2283804 22 21820335 1.336 |
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145 | eigbestsnp 4 rs2267006 22 21820990 1.336 |
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146 | eigbestsnp 4 rs6003566 22 21889806 1.000 |
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147 | eigbestsnp 4 rs16997606 22 21794754 0.875 |
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148 | eigbestsnp 4 rs5751611 22 21896019 0.823 |
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149 | eigbestsnp 4 rs5759636 22 21868698 0.799 |
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150 | eigbestsnp 4 rs2267000 22 21785366 0.584 |
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151 | packedancestrymap output |
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152 | ##end of smartpca run |
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153 | |
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154 | Correlation between eigenvector 1 (of 4) and Case/Control status is 0.033 |
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155 | Correlation between eigenvector 2 (of 4) and Case/Control status is 0.051 |
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156 | Correlation between eigenvector 3 (of 4) and Case/Control status is 0.193 |
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157 | Correlation between eigenvector 4 (of 4) and Case/Control status is -0.069 |
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158 | </pre></div>If you need to rerun this analysis, the command line used was |
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159 | smartpca.perl -i /opt/galaxy/test-data/tinywga.bed -a /opt/galaxy/test-data/tinywga.bim -b /opt/galaxy/test-data/tinywga.fam -o rgEigPCAtest1_pca.xls -p rgEigPCAtest1_eigensoftplot.pdf -e rgEigPCAtest1_eval.xls -l rgEigPCAtest1_log.txt -k 4 -m 2 -t 2 -s 2 |
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160 | <p/></div></body></html> |
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